Human recombinant bone morphogenetic protein-2 (rhBMP-2) has been proven effective in stimulating the regeneration of bone in both skeletal and extraskeletal locations. Through encapsulation within, and release from, biodegradable poly(DL-lactic-co-glycolic acid) (PLGA) microspheres, a proven vehicle for sustained delivery of various proteins, the local concentrations of rhBMP-2 could be maintained at optimal levels to stimulate bone regeneration and remodeling at the site of healing in diverse clinical settings. Thus the purpose of this work was to investigate the encapsulation of rhBMP-2 in PLGA microspheres and its biologic activity upon release. Using in vitro tests in simulated body fluids, the effect of rhBMP-2 released from PLGA microspheres upon osteoblast cell cultures was found to be statistically similar to the effect produced by positive controls consisting of nonencapsulated aqueous rhBMP-2 in simulated body fluids. This clarifies an important step in skeletal tissue engineering strategies aimed at the use of encapsulated rhBMP-2 to stimulate bone regeneration and remodeling. [S0148-0731(00)01303-0]
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June 2000
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Biological Activity of rhBMP-2 Released From PLGA Microspheres
J. B. Oldham,,
J. B. Oldham,
Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
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L. Lu,,
L. Lu,
Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
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X. Zhu, and,
X. Zhu, and
Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
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B. D. Porter,
B. D. Porter
Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
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T. E. Hefferan,
T. E. Hefferan
Department of Protein Chemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
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D. R. Larson,
D. R. Larson
Department of Biostatistics, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
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B. L. Currier,
B. L. Currier
Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
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A. G. Mikos,
A. G. Mikos
Department of Bioengineering, Rice University, Houston, TX 77005
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M. J. Yaszemski
M. J. Yaszemski
Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
11
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J. B. Oldham,
Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
L. Lu,
Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
X. Zhu, and
Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
B. D. Porter
Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
T. E. Hefferan
Department of Protein Chemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
D. R. Larson
Department of Biostatistics, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
B. L. Currier
Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
A. G. Mikos
Department of Bioengineering, Rice University, Houston, TX 77005
M. J. Yaszemski
11
Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905
Contributed by the Bioengineering Division of THE AMERICAN SOCIETY OF MECHANICAL ENGINEERS. Manuscript received by the Bioengineering Division October 21, 1999; revised manuscript received February 6, 2000. Associate Technical Editor: R. Vanderby, Jr.
J Biomech Eng. Jun 2000, 122(3): 289-292 (4 pages)
Published Online: February 6, 2000
Article history
Received:
October 21, 1999
Revised:
February 6, 2000
Citation
Oldham, , J. B., Lu, , L., Zhu, and , X., Porter , B. D., Hefferan , T. E., Larson , D. R., Currier , B. L., Mikos , A. G., and Yaszemski, M. J. (February 6, 2000). "Biological Activity of rhBMP-2 Released From PLGA Microspheres ." ASME. J Biomech Eng. June 2000; 122(3): 289–292. https://doi.org/10.1115/1.429662
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